14.6 B12

14.6.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 185.9047892 1 <0.0001 ***
B12 3.8091867 1 0.05 ns
Study 0.0365968 1 0.8 ns
GA_samp 179.4754671 1 <0.0001 ***
B12:Study 0.0049316 1 0.9 ns
hPL (μg/ml)
Chisq Df Pr(>Chisq)
(Intercept) 30.5887086 1 <0.0001 ***
B12 2.8526532 1 0.09 ns
Study 5.9992359 1 0.01 *
GA_samp 2530.3063435 1 <0.0001 ***
B12:Study 0.0735092 1 0.8 ns
GH2 (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 450.473469 1 <0.0001 ***
B12 17.408827 1 <0.0001 ***
Study 2.300564 1 0.1 ns
GA_samp 1101.855895 1 <0.0001 ***
B12:Study 4.864689 1 0.03 *
Insulin (uU/ml)
model term df1 df2 F.ratio Chisq p.value
1 B12 1 Inf 6.029 6.029 0.01 *
3 Study 1 Inf 4.201 4.201 0.04 *
4 GA_samp 1 Inf 0.862 0.862 0.4 ns
2 B12:Study 1 Inf 0.207 0.207 0.6 ns
HOMA-IR
model term df1 df2 F.ratio Chisq p.value
1 B12 1 Inf 5.275 5.275 0.02 *
3 Study 1 Inf 8.443 8.443 0.004 **
4 GA_samp 1 Inf 0.411 0.411 0.5 ns
2 B12:Study 1 Inf 0.118 0.118 0.7 ns
Glucose (mmol/L)
model term df1 df2 F.ratio Chisq p.value
1 B12 1 Inf 1.026 1.026 0.3 ns
3 Study 1 Inf 42.937 42.937 <0.0001 ***
4 GA_samp 1 Inf 0.629 0.629 0.4 ns
2 B12:Study 1 Inf 0.004 0.004 1.0 ns

14.6.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)
Var Effect P
(Intercept) 13.31 (9.28, 19.07) <0.0001
B12 1.00 (1.00, 1.00) 0.0007
StudySTOP 0.98 (0.88, 1.08) 0.67
GA_samp 1.15 (1.13, 1.18) <0.0001
hPL (μg/ml)
Var Effect P
(Intercept) 1.74 (1.44, 2.10) <0.0001
B12 1.00 (1.00, 1.00) 0.010
StudySTOP 1.11 (1.05, 1.18) 0.0001
GA_samp 1.34 (1.33, 1.36) <0.0001
GH2 (ng/ml)
Var Effect P
(Intercept) 0.06 (0.05, 0.08) <0.0001
B12 1.00 (1.00, 1.00) <0.0001
StudySTOP 1.11 (0.97, 1.26) 0.13
GA_samp 1.30 (1.28, 1.32) <0.0001
B12:StudySTOP 1.00 (1.00, 1.00) 0.03
Insulin (uU/ml)
Var Effect P
(Intercept) 14.19 (8.84, 22.76) <0.0001
B12 1.00 (1.00, 1.00) 0.01
StudySTOP 0.80 (0.70, 0.93) 0.002
GA_samp 1.01 (0.98, 1.04) 0.36
HOMA-IR
Var Effect P
(Intercept) 3.63 (2.20, 5.97) <0.0001
B12 1.00 (1.00, 1.00) 0.02
StudySTOP 0.69 (0.59, 0.80) <0.0001
GA_samp 1.01 (0.98, 1.04) 0.52
Glucose (mmol/L)
Var Effect P
(Intercept) 5.73 (5.24, 6.27) <0.0001
B12 1.00 (1.00, 1.00) 0.24
StudySTOP 0.86 (0.84, 0.88) <0.0001
GA_samp 1.00 (0.99, 1.00) 0.43

14.6.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

Results are averaged over the levels of B12 concentration (pM) and may be misleading when interaction term is involved.

PRL (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 99.71171 7.414826 84.56802 86.00620 115.6013
STOP 97.50398 6.918327 70.20794 84.63828 112.3254
hPL (μg/ml)
Study response SE df lower.CL upper.CL
SCOPE 89.06928 2.347226 147.7103 84.54950 93.83066
STOP 99.29534 2.386403 102.5592 94.67327 104.14307
GH2 (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 2.413961 0.0691488 461.2870 2.281830 2.553744
STOP 2.323107 0.0530918 224.8072 2.220807 2.430120
Insulin (uU/ml)
Study response SE df asymp.LCL asymp.UCL
SCOPE 14.66959 0.7087095 Inf 13.34428 16.12652
STOP 11.77008 0.4291986 Inf 10.95822 12.64208
HOMA-IR
Study response SE df asymp.LCL asymp.UCL
SCOPE 3.564459 0.1832610 Inf 3.222779 3.942365
STOP 2.463501 0.0947383 Inf 2.284643 2.656362
Glucose (mmol/L)
Study response SE df asymp.LCL asymp.UCL
SCOPE 5.487813 0.0513023 Inf 5.388178 5.589291
STOP 4.725790 0.0344336 Inf 4.658781 4.793763

14.6.4 Comparisons

Effect for every 100 unit increase in B12 concentration (pM).
Hormone Contrast Study Ratio of Geometric means (95% CI) P-value
PRL (ng/ml) B12366 / B12266 SCOPE 1.04 (1.02, 1.07) 0.0007
PRL (ng/ml) B12366 / B12266 STOP 1.04 (1.02, 1.07) 0.0007
hPL (μg/ml) B12366 / B12266 SCOPE 1.02 (1.00, 1.03) 0.010
hPL (μg/ml) B12366 / B12266 STOP 1.02 (1.00, 1.03) 0.010
GH2 (ng/ml) B12366 / B12266 SCOPE 1.07 (1.04, 1.11) <0.0001
GH2 (ng/ml) B12366 / B12266 STOP 1.03 (1.00, 1.05) 0.03
Insulin (uU/ml) B12366 / B12266 SCOPE 0.95 (0.92, 0.99) 0.01
Insulin (uU/ml) B12366 / B12266 STOP 0.95 (0.92, 0.99) 0.01
HOMA-IR B12366 / B12266 SCOPE 0.95 (0.92, 0.99) 0.02
HOMA-IR B12366 / B12266 STOP 0.95 (0.92, 0.99) 0.02
Glucose (mmol/L) B12366 / B12266 SCOPE 1.00 (0.99, 1.00) 0.2
Glucose (mmol/L) B12366 / B12266 STOP 1.00 (0.99, 1.00) 0.2

14.6.5 Estimated means (50 unit increase)

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

Results are averaged over the levels of B12 concentration (pM) and may be misleading when interaction term is involved.

PRL (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 98.66864 7.320598 83.80875 85.13344 114.3558
STOP 96.48401 6.841101 70.01038 83.76071 111.1400
hPL (μg/ml)
Study response SE df lower.CL upper.CL
SCOPE 88.68266 2.324569 144.7170 84.20516 93.39825
STOP 98.86434 2.371874 101.8153 94.26983 103.68279
GH2 (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 2.372818 0.0656375 411.5235 2.247237 2.505417
STOP 2.308809 0.0525050 220.9065 2.207618 2.414637
Insulin (uU/ml)
Study response SE df asymp.LCL asymp.UCL
SCOPE 14.84060 0.7124642 Inf 13.50788 16.30481
STOP 11.90729 0.4283918 Inf 11.09658 12.77723
HOMA-IR
Study response SE df asymp.LCL asymp.UCL
SCOPE 3.606633 0.1841795 Inf 3.263125 3.986302
STOP 2.492648 0.0946434 Inf 2.313885 2.685222
Glucose (mmol/L)
Study response SE df asymp.LCL asymp.UCL
SCOPE 5.492814 0.0506953 Inf 5.394346 5.593079
STOP 4.730096 0.0343693 Inf 4.663211 4.797941

14.6.6 Comparisons (50 unit increase)

Effect for every 50 unit increase in B12 concentration (pM).
Hormone Contrast Study Ratio of Geometric means (95% CI) P-value
PRL (ng/ml) B12316 / B12266 SCOPE 1.02 (1.01, 1.03) 0.0007
PRL (ng/ml) B12316 / B12266 STOP 1.02 (1.01, 1.03) 0.0007
hPL (μg/ml) B12316 / B12266 SCOPE 1.01 (1.00, 1.02) 0.010
hPL (μg/ml) B12316 / B12266 STOP 1.01 (1.00, 1.02) 0.010
GH2 (ng/ml) B12316 / B12266 SCOPE 1.03 (1.02, 1.05) <0.0001
GH2 (ng/ml) B12316 / B12266 STOP 1.01 (1.00, 1.02) 0.03
Insulin (uU/ml) B12316 / B12266 SCOPE 0.98 (0.96, 0.99) 0.01
Insulin (uU/ml) B12316 / B12266 STOP 0.98 (0.96, 0.99) 0.01
HOMA-IR B12316 / B12266 SCOPE 0.98 (0.96, 1.00) 0.02
HOMA-IR B12316 / B12266 STOP 0.98 (0.96, 1.00) 0.02
Glucose (mmol/L) B12316 / B12266 SCOPE 1.00 (1.00, 1.00) 0.2
Glucose (mmol/L) B12316 / B12266 STOP 1.00 (1.00, 1.00) 0.2

14.6.7 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

14.6.8 Plots